Biochemical and Hematological Parameters of Blood of Small Cattle when Feeding a New Feed Additive on Fattening

Author(s)

Serik N. Abdreshov , Makpal A. Yessenova , Georgiy A. Demchenko , Ulbossin N. Kozhaniyazova , Yerbulat K. Makashev , Anar N. Yeshmukhanbet , Аskar М. Kalekeshov1 , Laura U. Koibasova , Yerlan Е. Makashev ,

Download Full PDF Pages: 64-78 | Views: 11 | Downloads: 3 | DOI: 10.5281/zenodo.17471467

Volume 9 - October 2025 (10)

Abstract

The purpose of this study is to evaluate the physiological and biochemical efficacy of a new feed additive and its use based on laboratory studies. The use of a new feed additive has an effect on protein, carbohydrate and fat metabolism in the body, contributes to an increase in animal weight. The feed additive has a good adsorption and antioxidant effect, reduces the amount of toxic products, participates in the inactivation of free radicals and has a protective effect on cell membranes. It has been investigated that the use of a new feed additive leads to a significant improvement in growth and reduces lipid peroxidation, namely, it reduces the content of malondialdehyde and diene conjugates in the blood, and the most positive effect of these complexes on the protein and carbohydrate metabolism of sheep of both groups during feeding has been established.

Keywords

antioxidant, blood, feed additive, metabolism, Sheep

References

Abdreshov, S.N., Bulekbaeva, L.E., Demchenko, G.A. (2015). Lympho- and Hemodynamics in Dogs with Actue Experimental Pancreatitis. Bulletin of Experimental Biology and Medicine. 159: Р.32-34. DOI 10.1007/s10517-015-2882-0

Abdreshov, S.N., Bekezhan, M.A. 2019. Influence of feed addition on biochemical indexes of animals. J. Almaty Technological University, 4(125): P. 48-54.

Abdreshov, S.N., Ibraikhan, А.T., Alayev, I.Kh. (2018). The effect of toxicants on the membrane hydrolysis of the digestive tract in animals. News of the National Academy of Sciences of the Republic of Kazakhstan, Series of Agricultural sciences, 6(48): Р.24-31. https://doi.org/10.32014/2018. 2224-526Х.15

Alem, W.T. (2024). Effect of herbal extracts in animal nutrition as feed additives. Heliyon 10: e24973.  https://doi.org/10.1016/j.heliyon.2024.e24973

Alipour F., Vakili A., Mesgaran M.D., Ebrahimi H., (2019). The effect of adding ethanolic saffron petal extract and vitamin E on growth performance, blood metabolites, and antioxidant status in Baluchi male lambs. Asian Australas. J. Anim. 32: 1695–1704, doi:10.5713/ajas.18.0615

Artuso-Ponte, V., Pastor, A., Andratsch, M. (2020). The effects of plant extracts on the immune system of livestock. In book:  Feed Additives, doi: 10.1016/B978-0-12-814700-9.00017-0

Asres, A., Amha, N. (2014). Physiological Adaptation of Animals to the Change of Environment: A Review. J. Biol. Agric. Healthc. 4: 2224–3208.

Baker, H., Schor, S.M., Murphy, B.D., DeAngelis, B., Feingold, S., Frank, O. (1986). Blood vitamin and choline concentrations in healthy domestic cats, dogs, and horses. Am. J. Vet. Res. 47: 1468–1471

Bakhtiyarova, Sh., Kapysheva, U., Makashev, Y., Zhaksymov, B., Makashev, Y., Kalekeshov, A., Junussova, A., Bimenova, Zh. (2025). Novel Feed Mixture from Non-Traditional Forage Plants for Young Farm Animals. International Journal of Agriculture and Biosciences, https://doi.org/10.47278/journal.ijab/2025.108

Behn, C., Araneda, O.F., Celedón, G., González G. (2007). Hypoxia-related lipid peroxidation: evidences, implications and approaches. Respir Physiol Neurobiol. 158(2-3): 143-150. https://doi.org/10.1016/j.resp.2007.06.001

Berihulay, H., Abied, A., He, Xi., Jiang, L., Ma, Yu. (2019). Adaptation Mechanisms of Small Ruminants to Environmental Heat Stress. Animals (Basel). 28; 9(3):75. doi: 10.3390/ani9030075

Bhati, M., Dhuria, R., Sharma, T., Meel, M., Saini, S., (2017). Effect of Aloe vera as herbal feed additive on digestibility of nutrients and rumen fermentation in Rathi calves. Vet. Pract. 18: 282–283. https://cabidigitallibrary.org by 195.82.20.229, on 09/18/25.

Brassard, M.-È., Chouinard, P.Y., Tremblay, G.F., Gervais, R., Pouliota, É., Tessier, L., Gariépy, C., Cinq-Mars, D. (2024). Growth performance, carcass characteristics, and meatquality in meat and dairy goat kids fed aconcentrate-based diet or allotted to an intensiverotational grazing system. Can. J. Anim. Sci. 104: 488–502.  dx.doi.org/10.1139/cjas-2023-0075

Bostami, A.R., Khan, M.R.I., Rabbi, A.Z., Siddiqui, M.N., Islam, M.T., (2021). Boosting animal performance, immune index, and antioxidant status in post-weaned bull calves through dietary augmentation of selective traditional medicinal plants. Vet. Anim. Sci. 14: 100197, https://doi.org/10.1016%2Fj.vas.2021.100197

Boyko, T., Chaunina, E., Buzmakova, N., Zharikova, E., (2021). Biologically active additives for cows as a factor in the production of environmentally friendly products in animal husbandry. IOP Conf. Ser.: Earth. Environ. Sci. 624: https:// doi.org/10.1088/1755-1315/624/1/012063

Caprarulo, V., Ventura, V., Amatucci, A., Ferronato, G., Gilioli, G., (2022). Innovations for reducing methane emissions in livestock toward a sustainable system: analysis of feed additive patents in ruminants. Animals 12: 2760, https://doi.org/10.3390/ ani12202760

Chan, C.-L., Gan, R.-Y., Shah, N.P., Corke, H., (2018). Polyphenols from selected dietary spices and medicinal herbs differentially affect common food-borne pathogenic bacteria and lactic acid bacteria. Food Control. 92: 437–443, http://dx.doi.org/10.1016/j.foodcont.2018.05.032.

Gelaye, Y. (2024). Application of nanotechnology in animal nutrition: Bibliographic review. Animal Husbandry & Veterinary Science, 10(1): 2290308. https://doi.org/10.1080/23311932.2023.2290308

Gomez-Miranda, A., Estrada-Flores, J.G., Morales-Almaraz, E., Lopez-González, F., Flores-Calvete, G., Arriaga-Jordan, C.M. (2020). Barley or black oat silages in feeding strategies for small-scale dairy systems in the highlands of Mexico. Can. J. Anim. Sci. 100: 221–227. dx.doi.org/10.1139/cjas-2018-0237

Guo, X., Cheng, L., Liu, J., Zhang, S., Sun, X., Al-Marashdeh, O., (2019). Effects of licorice extract supplementation on feed intake, digestion, rumen function, blood indices and live weight gain of Karakul sheep. Animals 9: 279, https://doi.org/10.3390/ani9050279

Demchenko, G.A., Makashev, E.K., Bachtiyarova, Sh.K., Abdreshov, S.N., Koibasova, L.U. (2022). Biochemical applications and application of indicators in blood and lymph after a new feed additive based on bentonite. J. of Food Science and Nutrition Therapy. -USA: Peertechz Publications Pvt. 8(1): 01-05. https://doi.org/10.17352 /jfsnt.000031

Igbokwe, N.A. and Igbokwe, I.O. (2016a). Phenotypic variations in osmotic lysis of Sahel goat erythrocytes innon-ionic glucose media. J Basic ClinPhysiolPharmacol. 27(2): 147-154. https://doi.org/10.1515/jbcpp-2015-0036

Jo. C., Ahn, D.U. (1998). Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey. PoultSci. 77(3): 475-80. https://doi.org/10.1093/ps/77.3.475

Kiczorowska, B., Samolińska, W., Al-Yasiry, A., Kiczorowski, P., Winiarska-Mieczan, A. (2017). The natural feed additives as immunostimulants in monogastric animal nutrition – a review. Annals of Animal Science. 17(3): 605-625.

Li, J., Savransky, V., Nanayakkara, A., Smith. P.L., O’Donnell C.P, Polotsky, V.Y. (2007). Hyperlipidemia and lipid peroxidation depend on the truth of chronic intermittent hypoxia. J. Appl. Physiol. 102:557–563. https://doi.org/10.1152/japplphysiol.01081.2006

Mobashar, M. (2024). Understanding Concepts of Feed Additives in Animal Nutrition: their Potential in Improving Livestock Production Performance and Product Quality // Complementary and Alternative Medicine: Feed Additives. Unique Scientific Publishers, Faisalabad, Pakistan, 1-8.  DOI: 10.47278/book.CAM/2024.314

Muñoz-Cuautle, A., Ortega-Cerrilla, M.E., Herrera-Haro, J.G., Nava-Cuellar, C., Gutiérrez-Olvera, C., Ramírez-Bribiesca, J.E., Zetina-Córdoba, P., (2022). Effect of Oregano (Lippia graveolens) Essential oil as a phytogenic feed additive on productive performance, ruminal fermentation, and antioxidant activity in lamb meat. Agriculture 12: 973, https://doi.org/10.3390/agriculture12070973

Najjar, R.S. (2023). The Impacts of Animal-Based Diets in Cardiovascular Disease Development: A Cellular and Physiological Overview. J Cardiovasc Dev Dis. 30; 10(7):282. doi: 10.3390/jcdd10070282

Nanji, A.A., French, S.W., Mendenhall, C.L. (1989). Serum aspartate aminotransferase to alanine aminotransferase ratio in human and experimental alcoholic liver disease: relationship to histologic changes. Enzyme. 41(2): 112-5. doi: 10.1159/000469062.

Nastoh, N.A., Waqas, M., Çınar, A.A., Salman, M. (2024). The impact of phytogenic feed additives on ruminant production: A review. Journal of Animal and Feed Sciences, 33(4): 431–453, https://doi.org/10.22358/jafs/191479/2024

Neijat Mohamed, James D. (2024). House Safety and efficacy of hemp-derived products in animal feeds - a narrative review. Can. J. Anim. Sci. 104: 390–410. dx.doi.org/10.1139/cjas-2023-0133

Nigmatyanov, A.A., Nafikova, E.Z., Fedoseeva, N.A., Rybchenko, T.V., Kontsevaya, S.Yu., Pogodaev, V.A. (2020). The composition and properties of young cattle nutrition enriched with the additive “Tanrem. IOP Conf. Series: Earth and Environmental Science 613: 012091 doi:10.1088/1755-1315/613/1/012091

Pandey, A.K., Kumar, P., Saxena, M., (2019). Feed additives in animal health. In: R.C. Gupta, A. Srivastava, R. Lall (Editors). Nutraceuticals in Veterinary Medicine. Springer Nature. Cham (Switzerland), 345–362, http://dx.doi.org/10.1007/978-3-030-04624-8_23

Pappas, N.J.Jr., Qureshi, A.R. (1988). Liver aspartate aminotransferase activity as a power function of body weight. Biochem Med Metab Biol. 39(1): 121-5. doi: 10.1016/0885-4505(88)90067-9.

Penha-Silva, N., Firmino, C.B., de Freitas Reis, F.G.,  et al. (2007). Influence of age on the stability of human erythrocyte membranes. Mech Ageing Dev. 128: 444-449. https://doi.org/10.1016/j.mad.2007.06.007

Placha, I., Gai, F., Simonova, M.P. (2022). Editorial: Natural feed additives in animal nutrition—Their potential as functional feed. Front. Vet. Sci. 9: 1062724. doi: 10.3389/fvets.2022.1062724

Piao, M., Tu, Y., Zhang, N., Diao, Q., Bi, Y., (2023). Advances in the application of phytogenic extracts as antioxidants and their potential mechanisms in ruminants. Antioxidants 12: 879, https://doi.org/10.3390/antiox12040879

Ronald, J.T., Kendall C.S. (2019). Effects of dietary supplement sources on the rate and extent of in vitro ruminal degradation of alfalfa-based diets for cattle. Can. J. Anim. Sci. 100: 244–252 (2020) dx.doi.org/10.1139/cjas-2019-0105

Salvagno, G.L., Demonte, D., Dima, F., Bovo, Ch. Lippi, G. (2020). Stability of refrigerated whole blood samples for osmotic fragility test. Hematology, Transfusion and Cell Therapy. 42(2): 134-138. https://doi.org/10.1016/j.htct.2019.06.001

Shastak, Y., Pelletier, W. (2024). Pet Wellness and Vitamin A: A Narrative Overview. Animals (Basel). 14(7): 1000. doi: 10.3390/ani14071000

Shedeed, H.A., Farrag, B., Elwakeel, E.A., Abd El-Hamid, I.S., El-Rayes, M.A.-H., (2019). Propolis supplementation improved productivity, oxidative status, and immune response of Barki ewes and lambs. Vet. World 12: 834–843, https://doi.org/10.14202/vetworld.2019.834-843

Silveira, R.F., Roque-Borda, C.A., Vicente E.F., (2021). Antimicrobial peptides as a feed additive alternative to animal production, food safety and public health implications: An overview. Anim. Nutr. 7, 896–904, https://doi.org/10.1016/j.aninu.2021.01.004

Singh, P.K., (2015). An overview of feed additives. Animal Feed Additives. New India Publ. Age. New Delhi (India), 1–13, https://doi.org/10.59317/9789389130515

Tokayev, K.K. (2021). Unity of the people and systemic reforms are a solid foundation for the country's prosperity. URL: https://www.akorda.kz

Tokayev, K.K. (2019). Kazakhstan can increase agricultural revenues by $6 billion. URL: http:// finreview.info/ru. (date of access: 12/19/2019)

Tokayev, K.K. (2021). National project for the development of the agro-industrial complex of the Republic of Kazakhstan for 2021-2025. Concepts for the development of the agro-industrial complex of the Republic of Kazakhstan for 2021-2030 dated December 30, 2021 № 960

Walski, T., Chludzińska, L., Komorowska, M., Witkiewicz, W. (2014). Individual osmotic fragility distribution: a new parameter for determination of the osmotic properties of human red blood cells. BioMed research international. 162102. https://doi.org/10.1155/2014/162102

Wasowicz, W., Nève, J., Peretz, A., (1994). Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: Importance of extraction pH and influence of sample preservation and storage. Clinical Chemistry. 39(12): 2522-2526. https://doi.org/10.1093/clinchem/39.12.2522

Waugh, T., Asherman, E. (1938). The Use of An Index of Hemolysis in Expressing the Fragility of Erythrocytes. J. Lab. Clin. Med. (7): 746-751.

Yang, A. (2023). Benefits and Risks of Feed Additives in Animal Nutrition // Journal of Veterinary Science & Medical Diagnosis. 12: 2. DOI: 10.35248/2325-9590.23.12.100050.

Cite this Article: