Inhibitory Effect of the Bioagents Trichoderma spp. And Plant Extracts in Vitro on Rhizoctonia Solani
Author(s)
Jose Francisco Diaz-Najera , Sergio Ayvar-Serna , Mateo Vargas-Hernandez , Antonio Mena-Bahena , Omar G. Alvarado-Gomez , Refugia Vargas-Nava ,
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Abstract
Chili pepper cultivars are highly affected by pathogenic soil fungi causing wilting and reducing yields. As known, the use of fungicides to control these pathogens generates resistance, therefore biological and organic alternatives were evaluated. The objective of this study were to evaluate different Trichoderma strains, and organic products against the Rhizoctonia solani. In vitro conditions, were evaluated control with Trichoderma spp. and phytoextracts where diameter of colony, growth rate percentage and inhibition was evaluated. Statistical analysis showed highly significant differences (P<0.0001), T. harzianum and Trichoderma sp., inhibited the pathogen at 85.6 and 58.6%. Azadirachta indica+Cinnamomum spp. inhibited 100%, Lippia spp., Capsicum spp.+Cinnamomum spp., Cinnamomum spp.+Piper nigrum and Allium sativum inhibited 68, 65.2, 63.3 and 58.4%, respectively. T. harzianum, Trichoderma sp. and Azadirachta indica+Cinnamomum spp. showed the highest inhibition capacity.
Keywords
Biocontrol, plant extracts, soil fungy
References
i. Aguilar-Meléndez, A., Morrell, P. L., Roose, M. L., Kim, S. C. 2009. Genetic diversity and structure in semiwild and domesticated chiles (Capsicum annuum; Solanaceae) from Mexico. American Journal of Botany 96(6): 1190–1202. 2009.
ii. Al-Askar, A. A. and Rashad, Y. M. 2010. Efficacy of some plant extracts against Rhizoctonia solani on pea. Journal of Plant Protection Research 50(3): 239-243.
iii. Ali, H. Z. and Nadarajah, K. 2013. Evaluating the Efficacy of Trichoderma Isolates and Bacillus subtilis as Biological Control Agents against Rhizoctonia solani. Reseaech Journal of Applied Sciences 8(1): 72-81.
iv. Asad, S. A., Ali, N., Hameed, A., Ali, K. S., Ahmad, R., Bilal, M., Shahzad, M., Tabassum, A. 2014. Biocontrol efficacy of different isolates of Trichoderma against soil borne pathogen Rhizoctonia solani. Polish Journal of Microbiology 63(1):95-103.
v. Aslam, A., Naz, F., Arshad, M., Qureshi, R., Rauf, C. A. 2010. In vitro antifungal activity of selected medicinal plant diffusates against Alternaria solani, Rhizoctonia solani and Macrophomina Mhaseolina. Pakistan Journal of Botany 42 (4): 2911-2919.
vi. Castillo-Reyes, F., Hernández-Castillo, F. D, Gallegos-Morales, G., Flores-Olivas, A, Rodríguez-Herrera, R., Aguilar, C. N. 2015. Efectividad in vitro de Bacillus y polifenoles de plantas nativas de México sobre Rhizoctonia solani. Revista Mexicana de Ciencias Agrícolas 6(3): 549-562.
vii. Centurión-Belotto, S. A., Aquino-Jara, A. S., Bozzano-Saguier, G. 2013. Extractos vegetales para el control alternativo del damping-off causado por Rhizoctonia solani en plántulas de tomate. Investigación Agraria. 15(1):23-29.
viii. Chávez, A. R. and Aquino-Jara, A. S. 2012. Control de los hongos del suelo Rhizoctonia sp., Fusarium sp. y Sclerotium sp. con extractos vegetales. Investigación Agraria. 14(1):17-23.
ix. Chen, L. H., Zhang, J., Shao, X. H., Wang, S. S., Miao, Q. S., Mao, X. Y., Ya-Ming, Z. Y. M., She, D. L. 2015. Development and evaluation of Trichoderma asperellum preparation for control of sheath blight of rice (Oryza sativa L.) Biocontrol Science and Technology 25(3):316-328.
x. Díaz-Nájera, J. F., Alvarado-Gómez, O. G., Leyva-Mir, S. G., Ayvar-Serna, S., Michel-Aceves, A. C., Vargas-Hernández, M. 2015. Identification and control of fungi causing fruits rot in pipiana pumpkin (Cucurbita argyrosperma Huber). African Journal of Agricultural Research. 10(11):1150-1157.
xi. Díaz-Nájera, J. F., Vargas-Hernández, M., Ayvar-Serna, S., Alvarado-Gómez, O. G., Solís-Aguilar, J. F., Durán-Ramírez, J. A., Díaz-Ceniceros, H. L., Hernández-Aguilar, A. 2014. Identificación morfológica y por PCR de Rhizoctonia solani kühn a partir de frutos de calabaza pipiana y su manejo en invernadero. Biotecnia. 16(3):17-21.
xii. Granke, L. L., Quesada-Ocampo, L., Lamour, K., Hausbeck, M. K. 2012. Advances in Research on Phytophthora capsici on Vegetable Crops in The United States. Plant Disease (95)11: 1588-1600.
xiii. Hatamleh, A. A., Bahkali, A. H., El-Sheshtawi, M., Elgorban, A. M., El-Metwally, M. A. 2014. Inhibitory influence of plant extracts on soli borne fungi infecting muskmelon (Cucumis melo L.). International Journal of Pharmacology 10(6): 322-327.
xiv. Hernández-Castillo, F. D, Castillo-Reyes, F., Gallegos-Morales, G., Rodríguez-Herrera, R., Aguilar-González, C. N. 2010. Lippia graveolen Sand, Carya illinoensis Organic Extracts and there in vitro Effect against Rhizoctonia Solani Kuhn. American Journal of Agricultural and Biological Sciences 5(3): 380-384.
xv. Hoyos-Carbajal, L., Chaparro, P., Abramsky, M., Chet, I., Orduz, S. 2008. Evaluation of Trichoderma spp. against Rhizoctonia solani and Sclerotium rolfsii under in vitro and greenhouse conditions. Colombian Journal 26(3): 451-458.
xvi. Infante, D., Martínez, B., González, N., Reyes, Y. 2009. Mecanismos de acción de Trichoderma frente a hongos fitopatógenos. Revista Protección Vegetal 24(1):14-21.
xvii. Kotasthane, A., Toshy, A., Renu, K., Onkar, V. R. 2015. In vitro antagonism of Trichoderma spp. against Sclerotium rolfsii and Rhizoctonia solani and their response towards growth of cucumber, bottle gourd and bitter gourd. European Journal of Plant Pathology 14(1): 523–543.
xviii. Patil, N. N., Waghmode, M. S., Gaikwad, P. S., Gajbhiye M. H., Gunjal, A. B., Nawani, N., Kapadnis, B. P. 2014. Potential of Microbispora sp. V2 as biocontrol agent against Sclerotium rolfsii, the causative agent of southern blight of Zea mays L. (Baby corn) in vitro studies. Indian Journal of Experimental Biology 52(1): 1147-1151.
xix. Segarra, G., Avilés, M., Casanova, E., Borrero, C., Trillas, I. 2013. Effectiveness of biological control of Phytophthora capsici in pepper by Trichoderma asperellum strain T34. Phytopathologia Mediterranea 52(1):77−83.
xx. SIAP-SAGARPA. 2016. Agricultura producción anual. Servicio de Información Agroalimentaria y Pesquera (SIAP)- Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA), Ciudad de México, D.F., México. Disponible en: http://www.siap.gob.mx/cierre-de-la-produccion-agricola-por-cultivo/ (Consultada 10 Feb 2016).
xxi. Statistical Analysis System (SAS Institute). 2015. SAS Inc. SAS user´s guide: Statistics. Release 6.03. Ed. SAS Institute in corporation, Cary, N.C. USA: Author.
xxii. Vásquez-López, A., Tlapal-Bolaños, B., Yáñez-Morales, M. J., Pérez-Pacheco, R., Quintos-Escalante, M. 2009. Etiology wilt 'chile de agua' (Capsicum annuum L.) in Oaxaca, Mexico. Mexican Journal of Phytopathology 32 (2): 127-134.
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