Effects of Extraction Methods on Allelopathic Activity of Mimosa pigraL. Leaf Extract
Author(s)
Do Tan Khang , Tran Thanh Men , Nguyen Van Ay , Tran Ngoc Quy ,
Download Full PDF Pages: 33-40 | Views: 529 | Downloads: 139 | DOI: 10.5281/zenodo.4040929
Abstract
Giant sensitive plant (Mimosa pigra L.) belongs to Fabacae family, is an invasive species containing biological compounds (allelopathic chemicals) to control other plants. This study was conducted to investigate the effect of different extraction methods on the phenolic, flavonoid, mimosinne contents and evaluation the ability to inhibit germination, growth of radish (Raphanus sativus L.) and leaf mustard (Brassica juncea) from Mimosa pigra leaf extract. The total phenolic content, flavonoid and mimosine concentrations were determined by spectrophotometric measurement, and the ability to inhibit growth was performed in a laboratory, on petri dishes in 5 days. The method of incubation for mimosa leaves in 60 minutes at a temperature of 50°C has the highest phenolic, flavonoid and mimosine concentrations of 6.63 mg, 1.86 mg and 1.89 mg. The 60-minute incubation method inhibited radish with the highest 21.44% of the root length, 38.01% of the shoot height but inhibited the lowest germination rate and dry weight. The mixture of leaf extract 10% and vinegar 0.1% inhibited completely germination, root length, shoot height, and the weight of radish and leaf mustard compared to the control. This result provides scientific literature on the potential ability of this plant to control weeds.
Keywords
Allelopathy, Brassica juncea, extraction method, Mimosa pigra L., Raphanus sativus L.
References
i. Englert, J., Weniger, B., Lobstein, A., Anton, R., Krempp E., Guillaume, D., and Leroy, Y. 1995. Triterpenoid saponins from Mimosa pigra. Journal of Natural Products, 58(8): 1265‐1269.
ii. Kathiresh, M., Suganya, P., and Saravanakumar, M. 2012. Bioactive compounds in Sesbania sesban flower and its antioxidant and antimicrobial activity. Journal of Research in Pharmacy, 5(1): 293–390.
iii. Kawsar, M.A. 2008. Identification and Quantification of Phenolic Acids in Macrotyloma uniflorum by Reversed Phase-HPLC. American Journal of Plant Physiology, 3: 165-172.
iv. Khang, D. T., Tuan, N. T., Men, T. T., Ay, N. V., Thi, N. P. A., and Nhung, H. T. 2019. Phytotoxic effects of aqueous extracts from Mimosa pigra L. on barnyardgrass (Echinochloa crus-galli). International Journal of Agriculture and Biological Sciences, 6: 66-71.
v. Khang, D.T., La, A.H., Pham, H.T.H., Phung, T.T., Nguyen, Q.V., Luong, M.T., Luong, M.T., Nguyen, Q.T., Truong, M.N., Tran, X.D., Tran, K.D. and Trung, K.H. 2016. Allelopathic activity of dehulled rice and its allelochemicals on weed germination. International Letters of Natural Sciences, 58: 1-10.
vi. Li, Z. H., Wang, Q., Ruan, X., Pan, C. D. and Jiang, D. A. 2010. Phenolics and plantallelopathy. Molecules. 2010(15): 8933 - 8952.
vii. Okonkwo, C.J., Njoku, O. U., Okonkwo, T. J. N., Afieroho, O. E., and Proksch, P. 2016. Two new acylated flavonol glycosides from Mimosa pigra L. leaves sub-family Mimosoideae. Future Journal of Pharmaceutical Sciences, 2(2): 71-75.
vii. Rita, W., Swantara, I., Astiti Asih, I., and Sinarsih, N. 2018. Antibacterial activity of Samanea saman leaf ethanol extract against Escherichia coli and Staphylococcus aureus and its total flavonoid and phenolic contents. Jurnal Kimia (Journal Of Chemistry), 121-127.
viii. Saeheng, S. 2012. Chemical constituents and antioxidant activity of Borussus flabellifer, Elaeis guineensis, Mimosa diplotricha and Mimosa pigra. Medicinal Chemistry & Drug Discovery, 3(1): 52-57.
ix. Tanaka, T. 2012. Efficacy of flavonoid for patients with Japanese Cedar Pollinosis. INTECH Open Access Publisher, 382-383.
Cite this Article: