Isolation and Identification of Microorganisms from Soil Enriched With Different Fertilizers in Field Cultivation with Rice
Author(s)
Dania Stephen Okhumata , Edukpe, E. I , Dania, M. I ,
Download Full PDF Pages: 08-18 | Views: 416 | Downloads: 119 | DOI: 10.5281/zenodo.5515322
Abstract
Microorganisms are able to convert fertilizers that contain nitrogen, phosphorous, potassium and minerals in the soil for their growth. Seven samples (Control, PU, GU+P&K, PU+P&K, GU+P&K+FYM, PU+P&K+FYM) of the post-planting soil of rice used for isolation and identification of bacteria and fungi. A total of five bacterial species were isolated from the experimental plots which include Dipiobacillus species, Streptococcus species, Streptobacillus species, Coccobaccillus species and Staphylococus species. Bacteria isolated were mainly rod and cocci in shape. Dipiobacillus spp and Streptobacillus spp have single arrangement and is Gram negative. Streptococcus spp and Coccobacillus spp have chain arrangement and is Gram positive. Staphylococcus spp are cluster in arrangement and is Gram positive. Total viable colony counts of the bacterial isolates ranged from 1.04 x 103 – 9.60 x 103 and 1.40 x 105 – 8.00 x 105. A total of thirteen genera of fungi were isolated from plots treated with different fertilizers. The majority of isolates were members of the genus Phoma. The combination of inorganic and organic fertilizers significantly (p<0.05) increased fungal counts over control (8.0x105) The control (zero application) had lower isolates due to no (fertilizer application) to the soil. The combination of inorganic and organic fertilizers significantly (p<0.05) increased fungal counts over control (8.0x105).
Keywords
Bacteria, biofertilizer, Fungi, inorganic fertilizer, Isolation
References
i Adebayo, K. (1997). Communication in agriculture, Green Links International Abeokuta pp 1-56. Agriculture Journal of Crop Improvement. 15(2):1-19.
ii Ahemad, M., Zaidi, A., Khan, S., Oves, M. (2009): Factors affecting the variation of microbial communities in different agro ecsystems.- In: Microbial Strategies for crop improvement. Springer, Berlin Heidelberg, 301.
iii Aira, M., Gomez-Brandon, M., Lazcano, C., Baath, E. and Dominguez, J. (2010). Plant genotype strongly modifies the structure and growth of maize rhizosphere microbial communities. Soil Biology and Biochemistry. 42; 2276-2281(Google Scholar).
iv Anastasi, H. Varese, G.C. and Marcheiso, V.F. (2005). Isolation and Identification of fungi communities in compost and vermicompost. Mycologia, 97(1): 33-44.
v Bao, Z, Ikunaga Y, Matsushita Y, Morimoto S, Takada-Hoshino Y. (2012). Combined analysis of bacterial, fungi and nematode communities in Andosolic agricultural soils in Japan. Microbes Environment. 27:72-79.
vi Berendsen, R.L., Pieterse, C.M.J. and Bakker, P. (2012). The rhizosphere microbiome and plant health.Trends Plant Science. 17: 478-486.
vii Bielinska, E.J., Mocek-Plociniak, A. (2012). Impact of the tillage systems on the soil enzymatic activity, Archives of Environmental Protection. 38(1): 75-82.
viii Brzezinska, M., and S Wlodarczyk, T., (2005). Transformations in interacellular redox enzymes (oxidoreductase) – ActaAgrophysica, Dissertations and Monographs 3: 11-28.
ix Czurak-Dainard, M. (2005). The long-term impact of manure application on soil microbial properties and nutrient cycling in Manitoba soils. Msc. Thesis. Department of Soil Science, University of MB, Winnipegs, MB.
x Gaddeyya, G, Niharikaps. B.P. and Kumar, P. (2012). Isolation and Identification of Soil Mycoflora in different crop fields at SalurMandal. ADVAPPL Science Research; 3:2020-2026.
xi Giovana, C.V., and Antonella, A. and Valeria, F.M. (2005). Isolation and identification of fungal community in compost and vemicompost, Mycology 97(1): 33-44.
xii Habib, M.A.B., Yusoff, S.M., Phang, K.J. and Mohamed, S. (1997). Nutritional values of chironomia lavae grown in palm oil mill effluent and algaculture. Aquaculture 158: 95-105.
xiii Jabber, B.M., Al-Silawi, R. and Al-Najjar, T. (2012). Isolation and molecular identification of Asmycetes in sediments and waters of the Gulf of Aqaba, Red Sea. Natural Science, 4(8): 555-561.
xiv Kieliszewska-Rokicka, B. (2001). Soil enzymes and their significance in the study of biological activity of the soil. In The microorganisms of the soil environment, 37-42.
xv Kowalska, J., (2016). Effect of fertilization and microbiological bio-stimulators on healthiness and yield of organic potato-progress, Plant Protection, 56:230-235.
xvi Lesile, J.F., and Summeral. (2006). The Fusarium Laboratory Manual Science pp.388.
xvii Maheswari, N.U, and Komalavalli, R. (2013). Diversity of Soil Fungi from Thirurarur District, Tamil Nadu, India. International Journal Current Microbial APP Science., 2:135-141.
xviii Makasaki, K. and Ohtaki, A. (2002). A simple numerical model for predicting meter decomposition in Federal Batch Composting Operation. Journal of Environmental Quality. 31: 997-1003.
xix Max, P. (2012). “Horizontal DNA transfer between bacteria in the environment”. Journal of health physics 67(30): 280-282.
xx Mulani, R.M and Turkmane, K.L. (2014). Diversity of hizospheric fungi of ceropegiabulbosa Var. bulbosaRoxb. Journal Global Bioscience. 3(4); 1089-1093.
xxi Niewiadomska, A. (2013). Assessment of the impact of fertilizer and co- inoculation on the process diazotophy, biological and chemical properties of soil land crop condition under clover and alfalfa cultivation publication. University of Life Sciences in Poznan 106. (in Polish).
xxii Ogbulie, J. N., Uwaezuoke, J. C. And Ogiehor, S. I. (1998). Introductory Microbiology Practical. Springfield Publishers, Nigeria, pp67-92.
xxiii Olowokere, F.A. Adesodum, J.K., Akintokun, P.O., Egbedokin, A.O. and Martins, M.S. (2013). Soil chemical properties, yield and nutrient uptake of maize as influenced by plant and animal based on organic amendments. Nigeria Journal of Soil Science 23(1): 209-216.
xxiv Parr, J., Hornick, S. and Kaufmman, D. (1994). Use of microbial inoculants and organic fertilizers in agricultural production. Proceedings of the International Seminar on the use of Microbial and Organic Fertilizers in Agricultural Production, Published by the food and fertilizer technology center, Taipei, Taiwan.
xxv Rabia, A., Tasneem, A. and Fazia, S. (2007). Association of fungi, baderal and actinomycetes with different compost. Pakistan Journal of Botany. 39(6):2141-2151.
xxvi Rebecca, L.J. Dhanalakshmi V. Sharmila S. Susithra G. Kumar S. and Bala S. (2012). Isolation, Identification and Characterization of Fungi from Rhizosphere Soil of Barleria Cristata. International Journal Horticultural Crop Science Research.2:1-6.
xxvii Robert, O.and Greenwood, M. (2003). Practical Food Microbiology, Machester Blackwell Publisher pp245.
xxviii Sartori, L., Camperio, C. A., Bulgheroni, M., and Castiello, U. (2013). Reaching and grasping behaviour in Macaca Fasciculars: a Kinematic Study. Experimental Brain Research 224:119-124.
xxix Schulz, H.N., and B.B. Jorgensen. (2001). Big bacteria. Annual Review Microbiology 55 105-137.
xxx Storm, I.M.L.D., Kristensen, N.B., and Raw, J. (1985). Fungi and Selected Mycotoxins Form Pre and Post Fermented Corn Silage. Journal of Applied Microbiology 104:1034-1041.
Cite this Article: