Isolation and Identification of Microorganisms from Soil Enriched With Different Fertilizers in Field Cultivation with Rice

Author(s)

Dania Stephen Okhumata , Edukpe, E. I , Dania, M. I ,

Download Full PDF Pages: 08-18 | Views: 420 | Downloads: 121 | DOI: 10.5281/zenodo.5515322

Volume 5 - August 2021 (08)

Abstract

Microorganisms are able to convert fertilizers that contain nitrogen, phosphorous, potassium and minerals in the soil for their growth. Seven samples (Control, PU, GU+P&K, PU+P&K, GU+P&K+FYM, PU+P&K+FYM) of the post-planting soil of rice used for isolation and identification of bacteria and fungi. A total of five bacterial species were isolated from the experimental plots which include Dipiobacillus species, Streptococcus species, Streptobacillus species, Coccobaccillus species and Staphylococus species. Bacteria isolated were mainly rod and cocci in shape. Dipiobacillus spp and Streptobacillus spp have single arrangement and is Gram negative. Streptococcus spp and Coccobacillus spp have chain arrangement and is Gram positive. Staphylococcus spp are cluster in arrangement and is Gram positive. Total viable colony counts of the bacterial isolates ranged from 1.04 x 103 – 9.60 x 103 and 1.40 x 105 – 8.00 x 105. A total of thirteen genera of fungi were isolated from plots treated with different fertilizers. The majority of isolates were members of the genus Phoma. The combination of inorganic and organic fertilizers significantly (p<0.05) increased fungal counts over control (8.0x105)  The control (zero application) had lower isolates due to no (fertilizer application) to the soil. The combination of inorganic and organic fertilizers significantly (p<0.05) increased fungal counts over control (8.0x105).

Keywords

Bacteria, biofertilizer, Fungi, inorganic fertilizer, Isolation

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