The Attitude In Vitro of 10 Ubiquitous Bacteria in Seawater Polluted by Benzene and Toluene
Author(s)
Afef Meftah , Nassiba Messaoudi , Foudil Khelifa , Abdallah Bourhane Djebar , Khaldoun Bachari ,
Download Full PDF Pages: 122-141 | Views: 276 | Downloads: 90 | DOI: 10.5281/zenodo.5150052
Abstract
This study focuses on specifying a number of ubiquitous non-fastidious bacteria isolated from urine hospitalized patients for their abilities to degrade benzene and toluene. Using the MALDI-Tof technique (Bruker Daltonics), these opportunist bacteria have been identified. The bacteria were inoculated and incubated in sterile water contaminated with pure benzene and toluene for 63 days, at room temperature with continuous oxygenation. Analysis by Gas Chromatography/ Mass Spectrometry (GC / MS) HP6890 / HP 5973 MS (Agilent Technologies) allowed us to determine the concentration of each hydrocarbon and their derivatives. The results showed that not only the strains were able to completely degrade benzene and toluene in a single derivative: cyclohexane after less than 30 days. But even reveals variations in cyclohexane concentrations from one strain to another. Results reveal also that the Consumption of toluene by our strains was easier than with benzene, up to complete removal of toluene derivative in both species S1687 and S1671. The 2 strains belonging to the family Moraxellaceae S1670 and S1671 degrade benzene faster with concentrations 0.0475 μg/μl and 0.0727 μg/μl respectively, While both strains S5 and S476 of the Enterobacteriaceae family, had consumed totally and more easily cyclohexane (benzene derivative) with the lowest concentrations 0.0316 μg/μl and 0.0449 μg/μl respectively, this research confirmed that benzene, toluene and their derivatives were completely consumed without producing other identifiable intermediate metabolites.
Keywords
Aromatic hydrocarbons, Benzene, Toluene, GC / MS HP6890 / HP 5973 MS (Agilent Technologies), MALDI-Tof (Bruker Daltonics), Ubiquitous bacteria.
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