Control of Hyaloperonospora brassica by Certain Aqueous Plant Extracts in Adjara
Author(s)
Otar Shainidze , Shakro Kanchaveli , Guram Chkubadze , Nodar Beridze , Shota Lamparadze ,
Download Full PDF Pages: 64-72 | Views: 513 | Downloads: 119 | DOI: 10.5281/zenodo.5150030
Abstract
Cabbage (Brassica oleracea L.) is the most important vegetable among Brassica crops in the world due to its nutritional and health benefits, attached by many harmful Pathogens. The main objective of this study was to identify and determine the main pathogens of Cabbage and their Control.Fungal cultures identification was based on macroscopic characteristics like colony morphology, colour, texture, shape and appearance were observed on potato dextrose agar (PDA) and microscopic characteristics like conidia shape, hyphen colour, concentric zone, and pigmentation. Against pathogen used 24 different plant species known for their medicinal value in traditional medicine, were cut into small pieces and dried at room temperature for one week. 19 species of fungi have been identified. 9 species of fungi involved in forming of consortium on leaves of Cabbage plants have been specified as well. Among the mycobionts found on cabbage, the most dangerous and harmful pathogen of which the dominant species is Hyaloperonospora brassicae.Among the 27 extracts evaluated, 8 extracts showed mycelia inhibition above 81.8-97.96%, 4 showed moderate effect showed non-antifungal activity against Hyaloperonospora brassicae.This may be due to the lack of antifungal compound in the above mentioned 8 extracts. Based on the overall analysis it is very clearly shown that Alium cepa,Stevia rebaudiana, Inula racemosa, Buxus colchica,Rubia coradifolia, Panax ginseng and Corylopsis sinensis extracts can be emerged as safe alternatives to replace chemical fungicides against pathogens.
Keywords
cabbage, pathogen, Hyaloperonospora brassicae, leaf extract, antifungal activity, inhibition effect
References
i Lee, J., Izzah, N.K., Jayakodi, M., Perumal, S., Joh, H.J., Lee, H.J., Lee, S.C., Park, J.Y., Yang, K.W., Nou, I.S. and Seo, J., 2015. Genome-wide SNP identification and QTL mapping for black rot resistance in cabbage. BMC plant biology, 15(1), p.32. PubMed https://www.ncbi.nlm.nih.gov/pubmed/25644124
ii Bérénice, A.Stéphane, B. Claudie, M. Anne, Q. Yves, H. & D. Silué, 2003. Evidence that a Leaf-Disk Test Allows Assessment of Isolate-Specific Resistance in Brassica oleracea Crops Against Downy Mildew (Peronospora parasitica. European Journal of Plant Pathology, 109, pp: 471–478. https://link.springer.com/article/10.1023/A:1024217223829
iii Bahcevandziev, K. Silva, M.C. Monteiro, A.A. 2015. Histological characterization of downy mildew (Hyaloperonosporabrassicae) resistance in Portuguese cabbage(Brassica oleracea var. tronchuda). European Journal of Plant Pathology 141: 199-207.https://www.researchgate.net/publication/276930964
iv Dickinson, C. H.&J. R. Greenhalgh, 1977. Host range and taxonomy of Peronospora on crucifers.Transactions of the British Mycological Society, 69, pp: 111–116. https://doi.org/10.1016/S0007-1536(77)80121-6Get
v Dickson, M. H.&R. Petzoldt, 1993. Plant age and isolate source affect expression of downy mildew resistance in broccoli. Hort Science, 28, pp: 730–731. https://doi.org/10.21273/HORTSCI.28.7.730
vi Göker, M .Voglmayr, H. Blázquez, G. G.&F. Oberwinkler, 2009. Species delimitation in downy mildews: the case of Hyaloperonospora in the light of nuclear ribosomal ITS and LSU sequences. Mycological Research, 113, pp: 308–325.PubMed https://www.ncbi.nlm.nih.gov/pubmed/19061957
vii Jensen, B., D. Hockenhull, J.&L. Munk, 1999. Seedling and adult plant resistance to downy mildew (Peronosporaparasitica) in cauliflower (Brassica oleraceaconvar. botrytis var. botrytis). Plant Pathology, 48,pp: 604–612.https://doi.org/10.1046/j.1365-3059.1999.00388.x
viii N. Astley, D. Crute, I.R. Boukema, I.W. Santos, M. Bahcevandziev, K. Silva-Dias,J.Monteiro, A. A., 1998. Differential resistance to Peronosporaparasitica and Albugo candida in Brassica oleracea. ActaHorticulturae, 459, pp: 357–361.https://library.wur.nl/WebQuery/wurpubs/307333
ix Moss, N. A. Crute, I. R.&J. A. Lucas, 1994. Laboratory production of oospores of Peronospora parasitica (crucifer downy mildew) and the recovery and characterization of sexual progeny from crosses between isolates with different host specificity. Plant Pathology, 43, pp: 713–725.https://doi.org/10.1111/j.1365-3059.1994.tb01611.x
x Shainidze, O.,2019.Analysis of the Diversity of Microscopic Fungi in the Soils of Adjara,Georgia. IOP Conference Series Earth and Environmental Science, 362:012074. DOI:10.1088/1755-1315/362/1/012074
xi Harris, C.A. Renfrew M.J. and M.W Woolridge, 2001. Assessing the risk of pesticide residues to consumers: Recentandfuture developments. Food Additives and Contaminat. 18, pp: 1124-1129. PubMed https://www.ncbi.nlm.nih.gov/pubmed/11761124
xii Mahesh, B. and S. Satish, 2008. Antimicrobial activity of some important medicinal plant against plant and human pathogens.World Journal of Agricultural Scien. 4, pp:839-843. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.441.9036&rep=rep1&type=pdf
xiii Arif, T. Bhosale, J.D. Kumar, N. Mandal, T.K.Bendre, R.S.Lavekar, G.S.R.Dabur, 2009. Natural products-antifungalagents derived from plants. J. Asian Natural Products Res. 11(7), pp: 621-638.PubMed https://www.ncbi.nlm.nih.gov/pubmed/20183299
xiv Samy, R.P. and S.Ignacimuthu, 2000. Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats in India. J. Ethnopharm, 69 pp: 63-71. PubMed https://www.ncbi.nlm.nih.gov/pubmed/10661885
xv Palombo, E.A. and S.J. Semple, 2001. Antibacterial activity of traditional medicinal plants. Ethnopharm.77: 151- 157.PubMed https://www.ncbi.nlm.nih.gov/pubmed/11535358
xvi Behera SK and MK Misra, 2005. Indigenous phytotherapy for genito-urinary diseasesused by the Kandha tribeof Orissa, India. J. Ethnopharmacol,102 pp: 319-325. PubMed https://www.ncbi.nlm.nih.gov/pubmed/16055290
xvii Govindarajan, R., M. Vijayakumar, M. Singh, C.H.V. Rao, A. Shirwaikar, A.K.,S. Rawat and P.Pushpangadan, 2006. Antiulcer and antimicrobial activity of Anogeissuslatifolia. J. Ethnopharmacol.,106: 57-61.PubMed https://www.ncbi.nlm.nih.gov/pubmed/16413714
xviii Foster, M. Mueller, G. Bills, 2004. Biodiversity of fungi. Inventory and monitoring methods [Book]. –Boston: Elsevier Academic Press.,pp: 67- 98. https://www.elsevier.com/books/biodiversity-of-fungi/foster/978-0-12-509551-8
xix Holmgren, P.,K. Holmgren, N.,H. and L. C. Barbett, 1990. Index herbariorum, Part. 1: The Herbaria of the World. 8th edn. Regnum vegetab. 120, pp: 1-683.https://github.com/tdwg/prior-standards/tree/master/index-herbariorum-part-i
xx Samy, R.,P. and S. Ignacimuthu, 2000. Antibacterial activity of some folklore medicinal plants used bytribals in Western Ghats in India. J. Ethnopharmacol., 69: 63-71.PubMed https://www.ncbi.nlm.nih.gov/pubmed/10661885
xxi Palombo, E.,A. and S.J. Semple, 2001. Antibacterial activity of traditional medicinal plants. Ethnopharmacol., 77: 51-157. PubMed https://www.ncbi.nlm.nih.gov/pubmed/11535358
xxii Kumaraswamy, Y., P.Cox, J. M. Jaspars, L. Nahar and S.D. Sarker, 2002. Screening seeds of Scottishplants for antibacterial activity. J. Ethnopharmacol., 83:73-77 PubMed https://www.ncbi.nlm.nih.gov/pubmed/12413709
xxiii Stepanovic, S., N. Antic, I. Dakic and M. Svabic- vlahovic, 2003. In vitro antimicrobial activity of propilis and antimicrobial drugs.Microbiol. Res.,158: 353-357.PubMed https://www.ncbi.nlm.nih.gov/pubmed/14717457
xxiv Bylka, W., M. Szaufer-Hajdrych, I. Matalawskan and O. Goslinka, 2004. Antimicrobial activity ofisocytisoside and extracts of Aquilegia vulgaris L.Lett. Appl. Microbiol., 39: 93-97. PubMed https://www.ncbi.nlm.nih.gov/pubmed/15189294
xxv Balandrin, M.,F. Klocke, J.,A. Wurtele, E.,S. and W., H.Bollinger, 1985. Natural plant chemicals: Sources of Industrial and Medicinal materials. Science 228: 1154-1160. PubMed DOI:10.1126/science.3890182
xxvi Suman, T. Prabhu, P., S.Narendra S., B. Kumar and O. P.Chaurasia, 2016.FUNGICIDAL PROPERTIES OF HIGH ALTITUDE MEDICINAL PLANT: AN ANTIFUNGAL SUBSTANCE FROM THE EXTRACTS OF SELECTED MEDICINAL PLANTS.International Journal of Advanced Research, Volume 4, Issue 1932. CrossRef http://dx.doi.org/10.21474/IJAR01/478
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