Genetic Diversity of Catharanthus Roseus Species Based on DNA Barcodes
Author(s)
Do Tan Khang , Nguyen Pham Anh Thi , Tran Gia Huy ,
Download Full PDF Pages: 53-59 | Views: 413 | Downloads: 126 | DOI: 10.5281/zenodo.5515336
Abstract
Catharanthus roseus is a medicinal plant which contains high amount of vincristine and vinblastine, two anticancer compounds. In this study, 12 Catharanthus roseus individuals, which collected from three provinces in Mekong delta, were evaluated for their genetic diversity. Total DNA was extracted from fresh leaves and three DNA barcodes candidates were amplified, sequenced and aligned. On the basis of phylogenetic analysis and genetic distance, three DNA markers indicated the narrow genetic relationship among C. roseus accessions, the difference of flower color were not reflected on phylogenetic groups. The inter-genetic distance of ITS, matK and trnS-G were high. Therefore, C. roseus species were authenticated from Jussiaea repens, another species with similar flower and leaves morphology. Such findings proposed a reliable method for identification of medicinal plants.
Keywords
Catharanthus roseus, DNA barcodes, genetic diversity, medicinal plants, plant identification
References
i. Arora, R. A. J. E. S. H., Malhotra, P., Mathur, A.K., Mathur, A., Govil, C.M. and Ahuja, P. S. (2010). Anticancer alkaloids of Catharanthus roseus: transition from traditional to modern medicine. Herbal Medicine: A Cancer Chemopreventive and Therapeutic Perspective. Jaypee Brothers Medical Publishers Pvt. Ltd, New Delhi, India, 292-310.
ii. CBOL Plant Working Group. (2009). A DNA barcode for land plants. Proc. Natl. Acad. Sci. U. S. A., 12794-12797.
iii. Dong, W., Liu, J., Yu, J., Wang, L. and Zhou, S. (2012). Highly variable chloroplast markers for evaluating plant phylogeny at low taxonomic levels and for DNA barcoding. PloS one, 7(4): e35071.
iv. El-Domyati, F. M., Ramadan, A. M., Gadalla, N. O., Edris, S., Shokry, A. M., Hassan, S. M., et al., (2012). Identification of molecular markers for flower characteristics in Catharanthus roseus producing anticancer compounds. Life Sci. J., 9(4): 5949-5960.
v. Felsenstein J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39:783-791.
vi. Guimaraes, G., Cardoso, L., Oliveira, H., Santos, C., Duarte, P. and Sottomayor, M. (2012). Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don. AoB Plants, 2012.
vii. Hebert, P. D., Cywinska, A., Ball, S. L. and DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proc. R. Soc. Lond. Series B: Biological Sciences, 270(1512), 313-321.
viii. Ibrahim, M., Nazar, N., Ilyas, M. and Mahmood, T. (2014). Assessment of genetic variability among selected species of Apocynaceae on the basis of rps 11 gene. J. Anim. Plant Sci, 24(4), 1266-1269.
ix. Kress, W. J. (2017). Plant DNA barcodes: applications today and in the future. J. Syst. Evol., 55: 291–307.
x. Kumar S., Stecher G., Li M., Knyaz C., and Tamura K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol. Biol. Evol., 35:1547-1549.
xi. Lichota, A. and Gwozdzinski, K. (2018). Anticancer Activity of Natural Compounds from Plant and Marine Environment. Int. J. Mol. Sci., 19(11): 3533. https://doi.org/10.3390/ijms19113533
xii. Liu, S. H., Hoch, P. C., Diazgranados, M., Raven, P. H. and Barber, J. C. (2017). Multi‐locus phylogeny of Ludwigia (Onagraceae): insights on infra‐generic relationships and the current classification of the genus. Taxon, 66(5): 1112-1127.
xiii. Mahadani, P., Sharma, G. D. and Ghosh, S. K. (2013). Identification of ethnomedicinal plants (Rauvolfioideae: Apocynaceae) through DNA barcoding from northeast India. Pharmacogn. Mag., 9(35): 255.
xiv. Makki, R. M., Saeedi, A. A., Khan, T. K., Ali, H. M. and Ramadan, A. M., 2019. Single nucleotide polymorphism analysis in plastomes of eight Catharanthus roseus cultivars. Biotechnol. Biotechnol. Equip., 33: 419-428.
xv. Naipospos, N. and Palupi, D. (2019). Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents. Biodiversitas, 20(10).
xvi. Nejat, N., Valdiani, A., Cahill, D., Tan, Y. H., Maziah, M. and Abiri, R. (2015). Ornamental exterior versus therapeutic interior of Madagascar periwinkle (Catharanthus roseus): the two faces of a versatile herb. Sci. World J., 2015.
xvii. Rogers, S. O. and Bendich, A. J. (1989). Extraction of DNA from plant tissues. In Plant molecular biology manual (pp. 73-83). Springer, Dordrecht.
xviii. Saadedin, S. M. (2018). Genetic diversity and alkaloids profile evaluation of Catharanthus roseus L. based on RAPD molecular markers and RP-HPLC analysis. Pakistan J. Biotechnol., 15(2): 541-551.
xix. Selvaraj, D., Sarma, R. K., Shanmughanandhan, D., Srinivasan, R., & Ramalingam, S. (2015). Evaluation of DNA barcode candidates for the discrimination of the large plant family Apocynaceae. Plant Syst. Evol., 301(4): 1263-1273.
xx. Tamura K. and Nei M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol., 10:512-526.
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