Preliminary Screening and Antimicrobial, Antifungal Activity of Hormophysa articulate from the Sea of Kien Giang Province, Vietnam

Author(s)

Truong Thi Phuong Thao , Tran Thanh Men , Nguyen Thuy An , Nguyen Thi Hoai Ngoc , Nguyen Thi Kim Hue ,

Download Full PDF Pages: 87-98 | Views: 284 | Downloads: 99 | DOI: 10.5281/zenodo.5734107

Volume 5 - October 2021 (10)

Abstract

The study aimed to evaluate the bioactivity of ethanol extract from Hormophysa articulata collected in the waters of Kien Giang province, Vietnam. Experimental results determined the presence of flavonoids, phenolics, saponins, and tannins in Hormophysa articulate extract. The total of polyphenol and flavonoid content in Hormophysa articulata was analyzed to be 26.73±0.06 mg GAE/g extract and 218.72±2.06 mg QE/g extract, respectively.  Hormophysa articulate extract also showed in vitro antioxidant activity through 4 investigation methods of DPPH, ABTS∙+, reducing power (RP), and TAC. Under H2O2 and PQ-induced oxidative stress conditions, fruit flies reared in the medium supplemented with Hormophysa articulata extract at a concentration of 1 mg/mL of feed had a longer mean lifespan, 50% survival time, and maximum lifespan than the control which used standard feed. Investigation on the antibacterial activity identified that Hormophysa articulata was resistant to four strains of bacteria, including Enterococcus faecalis, Bacillus cereus, Bacillus subtilis, and Listeria innocua. The survey result confirmed the highest ability to resist Enterococcus faecalis, compared to the other three strains. It can be concluded that Hormophysa articulata is capable of synthesizing bioactive compounds with antioxidant and antibacterial effects

Keywords

antibacterial, antioxidant, fruit fly, Hormophysa articulate

References

             i            Crapo J.D., “Biology of disease: free radicals and tissue injury”, Laboratory Investigation; a Journal of Technical Methods and Pathology, Vol. 47, Issue.5, pp.412-426, 1982.

           ii            Balboa E.M., Conde E., Moure A., Falque E., & Dominguez H., “In vitro antioxidant properties of crude extracts and compounds from brown algae”, Food Chem, Vol.138, Issue.2-3, pp.1764-1785, 2013.

         iii            Brown E.S., Allsopp P.J., Magee, P.J., Gill C.I., Nitecki S., Strain C.R., & McSorley E.M, “Seaweed and human health”, Nutr Rev, Vol.72, Issue.3, pp.205-216, 2014.

         iv            Seca, Ana M.L., and Diana CGA Pinto, "Overview on the antihypertensive and anti-obesity effects of secondary metabolites from seaweeds." Marine drugs, Vol.16, Issue.7, pp.237, 2018.

           v            Yang, C.F., Lai, S.S., Chen, Y.H., Liu, D., Liu, B., Ai, C., Wan, X.Z., Gao, L.Y., Chen, X.H. and Zhao, C., "Anti-diabetic effect of oligosaccharides from seaweed Sargassum confusum via JNK-IRS1/PI3K signalling pathways and regulation of gut microbiota", Food and Chemical Toxicology, Vol.131, pp.110562, 2019.

         vi            Jasuja N.D., Sharma S.K., Saxena R., Choudhary J., Sharma R. and Joshi, S.C., “Antibacterial, antioxidant and phytochemical investigation of Thuja orientalis leaves”, Journal of Medicinal Plants Research, Vol.7, Issue.25, pp.1886-1893, 2013.

       vii            Singleton V.L., Orthofer R. and Lamuela– Raventos R.M., “Analysis of total phenol and other oxydation substrates and antioxydants by means of Folin-Ciocalteu reagent”, Method in Enzymology, Vol.299C. Issue.1, pp. 152-178, 1999.

     viii            Zhishen J., T. Mengcheng and W. Jianming, “The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals”, Food chemistry, Vol.64, Issue.4, pp.555-559, 1999.

         ix            Sharma O.P. and Bhat T.K., “DPPH antioxydant assay revisited”, Food chemistry, Vol.113, Issue.4, pp. 1202-1205, 2009.

           x            Nikolaos N., Wang L.F., Tsimidou M. and Zhang H.Y., “Estimation of scavenging sctivity of phenolic compounds using the ABTS assay”, Journal of Agricultural and Food Chemistry, Vol.52, Issue.15, pp.4669-4674, 2004.

         xi            Prieto P., Pineda M., and Aguilar M., “Spectrophotometric Quantitation of Antioxydant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E”, Analytical Biochemistry, Vol.269, pp.337–341, 1999.

       xii            Zhu Q.Y., R.M. Hackman, J.L. Ensunsa, R.R. Holt and C.L. Keen, “Antioxidative activities of oolong tea”, Journal of Agricultural and Food Chemistry, Vol.50, Issue.23, pp.6929-6934, 2002.

     xiii            Gargano J.W., I. Martin, P. Bhandari, M.S. Grotewiel, “Rapid iterative negative geotaxis (RING): a new method for assessing age-related locomotor decline in Drosophila”, Experimental gerontology, Vol.40, Issue.5, pp.386–395, 2005.

      xiv            Hadacek F., & Greger H, “Testing of antifunal natural products: Methodologies, comparability of resilts and assay choice”, Phytochemical Analysis: An international journal of Plant Chemical and Biochemical techniques,Vol.11, Issue.3, pp.137-147, 2000.

        xv            Cho S.H., Kang S.E., Cho J.Y., Kim A.R., Park S.M., Hong Y.K., & Ahn D.H., “The antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts”, Journal of medicinal food, Vol.10, Issue.3, pp.479–485, 2007.

      xvi            Marimuthu J., Essakimuthu P., Narayanan J., Anantham B., Tharmaraj R.J.J.M. and Arumugam S., “Phytochemical characterization of brown seaweed Sargassum wightii”, Asian Pacific Journal of Tropical Disease, Vol.2, pp.S109-S113, 2012.

    xvii            Nakai M., Kageyama N., Nakahara K. and Miki W., “Phlorotannins as radical scavengers from the extract of Sargassum ringgoldianum”, Marine Biotechnology, Vol.8, Issue.4, pp.409-414, 2006.

  xviii            Akremi N., Cappoen D., Anthonissen R., Verschaeve L. and Bouraoui A., “Phytochemical and in vitro antimicrobial and genotoxic activity in the brown algae Dictyopteris membranacea”, South African Journal of Botany, Vol.108, pp.308-314, 2017.

      xix            Manach C., Scalbert A., Morand C., Rémésy C., & Jiménez L., “Polyphenols: food sources and bioavailability” The American journal of clinical nutrition, Vol.79, Issue.5, pp.727-747, 2004.

        xx            Ford L., Stratakos A.C., Theodoridou K., Dick J.T., Sheldrake G.N., Linton M., Corcionivoschi N. and Walsh P.J, “Polyphenols from Brown Seaweeds as a Potential Antimicrobial Agent in Animal Feeds”, ACS Omega, Vol.5, Issue.16, pp.9093-9103, 2020.

      xxi            Tajrin A., Chandha M.H., Mappangara S., Ruslin M., Samad R. and Akbar F.H, “Analysis of the Total Level of Flavonoids in the Brown Algae (Phaeophyceae) Extract as Analgesic and Anti-Inflammatory Drugs”, Pesquisa Brasileira em Odontopediatria e Clínica Integrada, Vol.20, 2020.

    xxii            Baihakki B., Feliatra F. and Wikanta T., “Extraction of Polyphenol From Sargassum SP. and Its Entrapment in the Nanochitosan”, Diss. Riau University, Doctoral dissertation, 2014.

  xxiii            Palanisamy S., Vinosha M., Manikandakrishnan M., Anjali R., Rajasekar P., Marudhupandi T., Manikandan R., Vaseeharan B. and Prabhu N.M., "Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum”, International journal of biological macromolecules, Vol.116, pp.151-161, 2018.

  xxiv            Palanisamy S., Vinosha M., Marudhupandi T., Rajasekar P. and Prabhu N.M., “Isolation of fucoidan from Sargassum polycystum brown algae: Structural characterization, in vitro antioxidant and anticancer activity”,  International journal of biological macromolecules, Vol.102, pp.405-412, 2017.

    xxv            Prasedya E.S., Martyasari N.W.R., Abidin A.S., Ilhami B.T.K., Padmi H., Widyastuti S., Sunarwidhi A.L. and Sunarpi H., “Antioxidant activity of brown macroalgae Sargassum ethanol extract from Lombok coast, Indonesia”, In IOP Conference Series: Earth and Environmental Science, Vol. 712, No. 1, p. 012038, 2021.

  xxvi            Indu H. and Seenivasan R., “In vitro antioxidant activity of selected seaweeds from southeast coast of India”,  International Journal of Pharmacy and Pharmaceutical Sciences, Vol.5, Issue.2, pp.474-484, 2013.

xxvii            Padalko V., Dzyuba V., Kozlova O., Sheremet H. and Protsenko O., “Zingiber officinale extends Drosophila melanogaster life span in xenobiotic-induced oxidative stress conditions”, Frontiers in Biology, Vol.13, Issue.2, pp.130-136, 2018.

xxviii            Zhang Y., Xu M., Hu C., Liu A., Chen J., Gu C., Zhang X., You C., Tong H., Wu M. and Chen P., “Sargassum fusiforme Fucoidan SP2 Extends the Lifespan of Drosophila melanogaster by Upregulating the Nrf2-Mediated Antioxidant Signaling Pathway”, Oxid Med Cell Longev, 2019.

  xxix            Alam K., T. Agua, H. Maven, R.Taie, K.S. Rao, I. Burrows, M.E. Huber & T.Rali,”Preliminary Screening of Seaweeds, Seagrass and Lemongrass Oil from Papua New Guinea for Antimicrobial and Antifungal Activity”, International Journal of Pharmacognosy, Vol.32, Issue.4, pp.396-399, 1994.

    xxx            Abdel-Raouf, N., Al-Enazi, N.M., Ibraheem, I.B. and Al-Harbie, R.M., “Antibacterial and anti-hyperlipidemic activities of the brown alga Hormophysa cuneiformis from Ad Dammam Seashore”, Journal of Applied Pharmaceutical Science, Vol.5, Issue.8, pp. 114-125, 2015.

  xxxi            Seenivasan R., Rekha M., Indu H., & Geetha S, “Antibacterial Activity and Phytochemical Analysis of Selected Seaweeds from Mandapam Coast, India”, 2012.

Cite this Article: