Growth and Inhibitory Effect of Bacillus subtilis Against Fungi of Tomato Plants
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Abstract
Tomato is one of the important economic vegetable crops which are attacked by several serious diseases such as leaf blight. Bacillus genera is most feasible biocontrol microorganism suppress several pathogens like Alternaria spp. The efficiency of the antagonistic' treated plant by strains was evaluated using an in vitro assay. Dual culture examination was performed to investigate the ability of antagonistic bacteria to inhibit the growth of leaf blight caused by Alternaria spp. The efficacy of antagonistic Bacillus subtilis showed that the bacterium effectively suppressed the development of Alternaria alternata and Alternaria sp. at 38.67 and 32.89 % respectively while chemical fungicide agent (mancozeb) could inhibit the pathogen at 38.89 %. The shake flask culture of B. subtilis in 7 formulas media was carried at laboratory with 200 rpm for 120 h at room temperature (30°C). The results showed that the cell density at 96 h has been found for all medium. The highest cell density of 3.6 x 108 CFU mL-1 was achieved from NGB medium. These findings support the potential use of B. subtilis for biological control of Alternaria spp. on tomato plants
Keywords
antagonistic bacteria, biological control, leaf blight, Bacillus subtilis, Alternaria spp.
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